The Flora Mission for Ecosystem Composition, Disturbance and Productivity

Global land use and climate variability alter ecosystem conditions - including structure, function, and biological diversity - at a pace that requires unambiguous observations from satellite vantage points. Current global measurements are limited to general land cover, some disturbances, vegetation leaf area index, and canopy energy absorption. Flora is a pathfinding mission that provides new measurements of ecosystem structure, function, and diversity to understand the spatial and temporal dynamics of human and natural disturbances, and the biogeochemical and physiological responses of ecosystems to disturbance. The mission relies upon high-fidelity imaging spectroscopy to deliver full optical spectrum measurements (400-2500 nm) of the global land surface on a monthly time step at 45 meter spatial resolution for three years. The Flora measurement objectives are: (i) fractional cover of biological materials, (ii) canopy water content, (iii) vegetation pigments and light-use efficiency, (iv) plant functional types, (v) fire fuel load and fuel moisture content, and (vi) disturbance occurrence, type and intensity. These measurements are made using a multi-parameter, spectroscopic analysis approach afforded by observation of the full optical spectrum. Combining these measurements, along with additional observations from multispectral sensors, Flora will far advance global studies and models of ecosystem dynamics and change

Functional magnetic nanoparticles for protein delivery applications : understanding protein-nanoparticle interactions

Iron oxide nanoparticles (IONPs) surface functionalised with thermo-responsive polymers can encapsulate therapeutic proteins and release them upon heating with an alternating magnetic field above the lower critical solution temperature (LCST). In order to make this delivery system clinically-relevant, we prepared IONPs coated with poly-N-isopropylmethacrylamide (PNIPMAM), a polymer with LCST above human body temperature. The optimal polymer chain length and nanoparticle size to achieve LCST of ca. 45 °C were 19 kDa PNIPMAM and 16 nm IONPs. The PNIPMAM-coated IONPs could encapsulate a range of proteins which were released upon heating above LCST in the presence of a competitor protein or serum. A small amount of encapsulated protein leakage was observed below LCST. The efficiency of protein encapsulation and release was correlated with molecular weight and glycosylation state of the proteins. Magnetic heating resulted in a faster protein release as compared to conventional heating without significant temperature increase of the bulk solution

Sensor Webs with a Service-Oriented Architecture for On-demand Science Products

This paper describes the work being managed by the NASA Goddard Space Flight Center (GSFC) Information System Division (ISD) under a NASA Earth Science Technology Ofice (ESTO) Advanced Information System Technology (AIST) grant to develop a modular sensor web architecture which enables discovery of sensors and workflows that can create customized science via a high-level service-oriented architecture based on Open Geospatial Consortium (OGC) Sensor Web Enablement (SWE) web service standards. These capabilities serve as a prototype to a user-centric architecture for Global Earth Observing System of Systems (GEOSS). This work builds and extends previous sensor web efforts conducted at NASA/GSFC using the Earth Observing 1 (EO-1) satellite and other low-earth orbiting satellites

EO-1/Hyperion: Nearing Twelve Years of Successful Mission Science Operation and Future Plans

The Earth Observing One (EO-1) satellite is a technology demonstration mission that was launched in November 2000, and by July 2012 will have successfully completed almost 12 years of high spatial resolution (30 m) imaging operations from a low Earth orbit. EO-1 has two unique instruments, the Hyperion and the Advanced Land Imager (ALI). Both instruments have served as prototypes for NASA's newer satellite missions, including the forthcoming (in early 2013) Landsat-8 and the future Hyperspectral Infrared Imager (HyspIRI). As well, EO-1 is a heritage platform for the upcoming German satellite, EnMAP (2015). Here, we provide an overview of the mission, and highlight the capabilities of the Hyperion for support of science investigations, and present prototype products developed with Hyperion imagery for the HyspIRI and other space-borne spectrometers

EO-1 Data Quality and Sensor Stability with Changing Orbital Precession at the End of a 16 Year Mission

The Earth Observing One (EO-1) satellite has completed 16 years of Earth observations in early 2017. What started as a technology mission to test various new advancements turned into a science and application mission that extended many years beyond the satellites planned life expectancy. EO-1s primary instruments are spectral imagers: Hyperion, the only civilian full spectrum spectrometer (430-2400 nm) in orbit; and the Advanced Land Imager (ALI), the prototype for Landsat-8s pushbroom imaging technology. Both Hyperion and ALI instruments have continued to perform well, but in February 2011 the satellite ran out of the fuel necessary to maintain orbit, which initiated a change in precession rate that led to increasingly earlier equatorial crossing times during its last five years. The change from EO-1s original orbit, when it was formation flying with Landsat-7 at a 10:01am equatorial overpass time, to earlier overpass times results in image acquisitions with increasing solar zenith angles (SZAs). In this study, we take several approaches to characterize data quality as SZAs increased. Our results show that for both EO-1 sensors, atmospherically corrected reflectance products are within 5 to 10 of mean pre-drift products. No marked trend in decreasing quality in ALI or Hyperion is apparent through 2016, and these data remain a high quality resource through the end of the mission

The HYSPIRI Decadal Survey Mission: Update on the Mission Concept and Science Objectives for Global Imaging Spectroscopy and Multi-Spectral Thermal Measurements

The NASA HyspIRI mission is planned to provide global solar reflected energy spectroscopic measurement of the terrestrial and shallow water regions of the Earth every 19 days will all measurements downlinked. In addition, HyspIRI will provide multi-spectral thermal measurements with a single band in the 4 micron region and seven bands in the 8 to 12 micron region with 5 day day/night coverage. A direct broadcast capability for measurement subsets is also planned. This HyspIRI mission is one of those designated in the 2007 National Research Council (NRC) Decadal Survey: Earth Science and Applications from Space. In the Decadal Survey, HyspIRI was recognized as relevant to a range of Earth science and science applications, including climate: "A hyperspectral sensor (e.g., FLORA) combined with a multispectral thermal sensor (e.g., SAVII) in low Earth orbit (LEO) is part of an integrated mission concept [described in Parts I and II] that is relevant to several panels, especially the climate variability panel." The HyspIRI science study group was formed in 2008 to evaluate and refine the mission concept. This group has developed a series of HyspIRI science objectives: (1) Climate: Ecosystem biochemistry, condition & feedback; spectral albedo; carbon/dust on snow/ice; biomass burning; evapotranspiration (2) Ecosystems: Global plant functional types, physiological condition, and biochemistry including agricultural lands (3) Fires: Fuel status, fire frequency, severity, emissions, and patterns of recovery globally (4) Coral reef and coastal habitats: Global composition and status (5) Volcanoes: Eruptions, emissions, regional and global impact (6) Geology and resources: Global distributions of surface mineral resources and improved understanding of geology and related hazards These objectives are achieved with the following measurement capabilities. The HyspIRI imaging spectrometer provides: full spectral coverage from 380 to 2500 at 10 nm sampling; 60 m spatial sampling with a 150 km swath; and fully downlinked coverage of the Earth's terrestrial and shallow water regions every 19 days to provide seasonal cloud-free coverage of the terrestrial surface. The HyspIRI Multi-Spectral Thermal instrument provides: 8 spectral bands from 4 to 12 microns; 60 m spatial sampling with a 600 km swath; and fully downlinked coverage of the Earth's terrestrial shallow water regions every 5 days (day/night) to provide nominally cloud-free monthly coverage. The HyspIRI mission also includes an on-board processing and direct broadcast capability, referred to as the Intelligent Payload Module (IPM), which will allow users with the appropriate antenna to download a subset of the HyspIRI data stream to a local ground station. These science and science application objectives are critical today and uniquely addressed by the combined imaging spectroscopy, thermal infrared measurements, and IPM direct broadcast capability of HyspIRI. Two key objectives are: (1) The global HyspIRI spectroscopic measurements of the terrestrial biosphere including vegetation composition and function to constrain and reduce the uncertainty in climate-carbon interactions and terrestrial biosphere feedback. (2) The global 8 band thermal measurements to provide improved constraint of fire related emissions. In this paper the current HyspIRI mission concept that has been reviewed and refined to its current level of maturity with a Data Products Symposium, Science Workshop and NASA HWorkshop is presented including traceability between the measurements and the science and science application objectives

Epithelial-to-mesenchymal transition drives a pro-metastatic Golgi compaction process through scaffolding protein PAQR11

Tumor cells gain metastatic capacity through a Golgi phosphoprotein 3-dependent (GOLPH3-dependent) Golgi membrane dispersal process that drives the budding and transport of secretory vesicles. Whether Golgi dispersal underlies the prometastatic vesicular trafficking that is associated with epithelial-to-mesenchymal transition (EMT) remains unclear. Here, we have shown that, rather than causing Golgi dispersal, EMT led to the formation of compact Golgi organelles with improved ribbon linking and cisternal stacking. Ectopic expression of the EMT-activating transcription factor ZEB1 stimulated Golgi compaction and relieved microRNA-mediated repression of the Golgi scaffolding protein PAQR11. Depletion of PAQR11 dispersed Golgi organelles and impaired anterograde vesicle transport to the plasma membrane as well as retrograde vesicle tethering to the Golgi. The N-terminal scaffolding domain of PAQR11 was associated with key regulators of Golgi compaction and vesicle transport in pull-down assays and was required to reconstitute Golgi compaction in PAQR11-deficient tumor cells. Finally, high PAQR11 levels were correlated with EMT and shorter survival in human cancers, and PAQR11 was found to be essential for tumor cell migration and metastasis in EMT-driven lung adenocarcinoma models. We conclude that EMT initiates a PAQR11-mediated Golgi compaction process that drives metastasis

Use of the Earth Observing One (EO-1) Satellite for the Namibia SensorWeb Flood Early Warning Pilot

The Earth Observing One (EO-1) satellite was launched in November 2000 as a one year technology demonstration mission for a variety of space technologies. After the first year, it was used as a pathfinder for the creation of SensorWebs. A SensorWeb is the integration of variety of space, airborne and ground sensors into a loosely coupled collaborative sensor system that automatically provides useful data products. Typically, a SensorWeb is comprised of heterogeneous sensors tied together with a messaging architecture and web services. Disasters are the perfect arena to use SensorWebs. One SensorWeb pilot project that has been active since 2009 is the Namibia Early Flood Warning SensorWeb pilot project. The Pilot Project was established under the auspices of the Namibian Ministry of Agriculture Water and Forestry (MAWF)/Department of Water Affairs, the Committee on Earth Observing Satellites (CEOS)/Working Group on Information Systems and Services (WGISS) and moderated by the United Nations Platform for Space-based Information for Disaster Management and Emergency Response (UN-SPIDER). The effort began by identifying and prototyping technologies which enabled the rapid gathering and dissemination of both space-based and ground sensor data and data products for the purpose of flood disaster management and water-borne disease management. This was followed by an international collaboration to build small portions of the identified system which was prototyped during that past few years during the flood seasons which occurred in the February through May timeframe of 2010 and 2011 with further prototyping to occur in 2012. The SensorWeb system features EO-1 data along with other data sets from such satellites as Radarsat, Terra and Aqua. Finally, the SensorWeb team also began to examine the socioeconomic component to determine the impact of the SensorWeb technology and how best to assist in the infusion of this technology in lesser affluent areas with low levels of basic infrastructure. This paper provides an overview of these efforts, highlighting the EO-1 usage in this SensorWeb

Health and environmental applications of gut microbiome: A review

Life on Earth harbours an unimaginable diversity of microbial communities. Among these, gut microbiome, the ecological communities of commensal, symbionts (bacteria and bacteriophages) are a unique assemblage of microbes. This microbial population of animal gut helps in performing organism's physiological processes to stay healthy and fit. The role of these microbial communities is immense. They continually maintain interrelation with the intestinal mucosa in a subtle equilibrium and help the gut for different functions ranging from metabolism to immunologic functions like upgradation of nutrient-poor diets, aid in digestion of recalcitrant food components, protection from pathogens, contribute to inter-And intra-specific communication, affecting the efficiency as disease vectors etc. The microbial diversity in the gut depends upon environmental competition between microbes, their sieving effects and subsequent elimination. Due to wide diversity of anatomy and physiology of the digestive tracts and food habits, the gut microbiome also differs broadly among animals. Stochastic factors through the history of colonization of the microbiome in a species and in situ evolution are likely to establish interspecies diversity. Moreover, the microbes offer enormous opportunity to discover novel species for therapeutic and/or biotechnological applications. In this manuscript, we review the available knowledge on gut microbiome, emphasising their role in health and health related applications in human. © 2017 Soumya Chatterjee et al., published by De Gruyter Open 2017